Tcherkez, Guillaumeīreast cancer is the most common cancer in women worldwide. Tea, Illa Martineau, Estelle Antheaume, Ingrid Lalande, Julie Mauve, Caroline Gilard, Francoise Barillé-Nion, Sophie Blackburn, Anneke C. TERRA abundance is associated with the stability of RNA in cervical cancer cells, but is unlikely associated with telomere length.ġ3C and 15N natural isotope abundance reflects breast cancer cell metabolism TERRA degrades rapidly in HeLa cells, but is maintained stably in other cervical cancer cells that accumulate higher levels of TERRA. On the whole, our data indicate that TERRA abundance and stability vary between different types of cervical cancer cells. Telomere length varied from 4 to 9 kb in the cells and did not correlate significantly with the TERRA level. An RNA stability assay of actinomycin D-treated cells revealed that TERRA had a short half-life of ~4 h in HeLa cells, which was consistent with previous studies, but was more stable with a longer half-life (>8 h) in the other 5 cell lines. We found that the TERRA level was several fold greater in the SiHa, CaSki, HeLa S3, C-33A and SNU-17 cells, than in the HeLa cells. We also examined the association between the TERRA level and its stability and telomere length. Thus, in this study, we measured TERRA levels and stability, as well as telomere length in 6 cervical cancer cell lines, HeLa, SiHa, CaSki, HeLa S3, C-33A and SNU-17. However, TERRA abundance and stability have not been examined in other cervical cancer cells, at least to the best of our knowledge. TERRA has been well characterized in HeLa cells, a type of cervical cancer cell. Telomeres are transcribed into long non-coding RNA, referred to as telomeric repeat-containing RNA (TERRA), which plays important roles in maintaining telomere integrity and heterochromatin formation. Oh, Bong-Kyeong Keo, Ponnarath Bae, Jaeman Ko, Jung Hwa Choi, Joong Sub Variable TERRA abundance and stability in cervical cancer cells. These studies begin to develop a framework for exploiting the oral microbiome for monitoring oral cancer development, progression and recurrence. Weighted UniFrac principal coordinates analysis based on 12 taxa separated most cancers from other samples with greatest separation of node positive cases. Significant decreases in abundance of these phyla were observed for pre- cancers, but not when comparing samples from contralateral sites (tongue and floor of mouth) from healthy individuals.
In cancer samples from both a discovery and a subsequent confirmation cohort, abundance of Firmicutes (especially Streptococcus) and Actinobacteria (especially Rothia) was significantly decreased relative to contralateral normal samples from the same patient. To investigate changes in the microbiome associated with oral cancers, we profiled cancers and anatomically matched contralateral normal tissue from the same patient by sequencing 16S rDNA hypervariable region amplicons. Individual bacteria and shifts in the composition of the microbiome have been associated with human diseases including cancer. Kuczynski, Justin Bhattacharya, Aditi Huey, Bing Corby, Patricia M. Changes in Abundance of Oral Microbiota Associated with Oral Cancer
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